![]() There is a chapter in the user guide that deals with this question. So please determine the relation between pixels and millimeters for the final camera setup. This is no option for automatic counting! Please study the ImageJ user guide and especially the chapters dealing with image formats, stacks, histograms, thresholding, measurements, and particle analyses.Ĭoncerning RAW-conversion I shall give you some advice in a separate post.Īlternative would be using Freehand Selection and Clear Outside. So please tell me for whom you are working and why you don’t get support for at least the minimum requirements. There are a lot of open questions on your side and you must think about the work I’ve already put into my consultancy. I did still not clearly comprehend, how can I counting my whiteflies using ImageJ? I have no experience with color adjustment, but I wiII try it and as well adjust the focal length. I would like to count only the adults, with a body length of 1 mm to 2mm. Whiteflies are small sap-sucking insects one of the most important agricultural pests. Alternative would be using Freehand Selection and Clear Outside. ![]() What can I use?Ī problem with the background is the easiest thing for me. RW2-files.ĭo you know any other software that would be able to open RAW images (.RW2-file), export them as 16 bit uncompressed TIFF-files? Software like XnView, IrfanView or Online are not suitable for this. Unfortunately, I don’t have an Apple computer and thus cannot open the. In fact, I thought it would be a Minolta camere. As mentioned earlier, long focal length optics are better suited here. The camera optics appear to be of the wide-angle type with a focal length of 11.9 mm which corresponds to 33 mm for a 35mm camera. It would be interesting to know how small a fly can be or what must be regarded as being a maggot.ĭon’t forget to experiment with colored light.Īlthough the images are already quite good, every (contrast) improvement will make the processing more robust. Here is an example of a thresholded image: A problem is the background which should be uniform matt black or matt gray. The raw images are of good quality and I’m sure that a promising processing scheme can be found. ![]() If you are on a Mac the conversion problem appears easy to solve. I’m on a Mac and the Preview application that comes with macOS is able to open the raw images and to export them as 16 bit uncompressed TIF-files that open in ImageJ as RGB-stacks. Don’t use these programs.īTW your RAW-images appear to be from a Panasonic camera, not a Minolta. Most of the images that come out of LR are quite good, excellent for making a proof set for a client, but they are by no means a finished image.Both converted images (reaConverter7 -RAW-to-TIF.tif, XnViewer-RAW-to-TIF.tif) are unsuited, the conversion applications apply a geometrical correction which is simply not applicable. Once you've taken your images as far as you can in Lightroom, you would be able to create truly finished images in PS. You also get a world-class bitmapped graphic editor as well - Photoshop 2018. Upgrade to Lightroom Classic CC 7.2 and stop messing around with temporary solutions. You are using software that is 2 versions old. I routinely go through my oldest DSLR images from 2006 and re-interpret them in the newest raw converters and I am often surprised, in a pleasant way, at how much better the look. If you choose to convert to dng you should keep that in mind, and NEVER toss your raw files. Being able to edit camera native raw files is always backwards compatible - in that once the raw converter has been updated to read a raw file from a newer camera, it will never stop doing that. The problem with saving your images as dng is that if you want to use a program to edit these files down the road that does not recognize dng, you are out of luck. My question is do I still the RW2 files when I already have the DNG files? Does the DNG file contain all the info in the original RW2 files? Thanks. My Lightroom 5.7.1 cannot read Panasonic RW2 files so I convert them first to Adobe DNG before importing to Lightroom.
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